Detection of femtogram amounts of biogenic amines using self-assembled DNA-protein nanostructures
نویسندگان
چکیده
Chimera Biotec, founded in 2000 as a spin-off from academic research in Germany, has implemented its proprietary DNA-antibody conjugates within the highly innovative Imperacer product line, enabling the ultra-sensitive detection of proteins and other antigens. The ready-to-use, commercially available Imperacer kits offer a performance-enhancing tool to improve the limit of detection of conventional ELISA protocols by more than 1,000-fold. Hence, the detection and quantification of trace amounts of basically any antigen, ranging from large proteins to small molecules such as hormones or biogenic amines, can be accomplished with an Imperacer-boosted immunoassay.
منابع مشابه
Biogenic amines determination in some traditional cheeses in West Azerbaijan province of Iran
Biogenic amines (BA) are nitrogenous compounds that possess biological activity. The source of production is the microbial decarboxylation of amino acids. This compounds are found in various types of cheese. The aim of this work was to evaluate the BA content of some traditional cheeses in West Azerbaijan province Iran. For this purpose, 70 samples of Koopeh, 10 samples of Lighvan and 5 samples...
متن کاملA novel approach to Au nanoparticle-based identification of DNA nanoarrays.
The combination of electron beam lithography and gold nanoparticle-based detection method is subject to a novel high-resolution approach to detecting DNA nanoarrays. In this work, gold nanoparticle-based detection of DNA hybridization on nanostructured arrays is presented. The nanostructured arrays were created by electron beam lithography of a self-assembled monolayer. Amine groups, which are ...
متن کاملRecent advances in self-assembled DNA nanosensors
Over the past 30 years DNA has been assembled into a plethora of structures by design, based on its reliable base pairing properties. As a result, many applications of DNA nanotechnology are emerging. Here, we review recent advances in the use of self-assembled DNA nanostructures as sensors. In particular, we focus on how defined nanostructures, such as rigid DNA tetrahedra, provide an advantag...
متن کاملShRNA-mediated knock-down of CD200 using the self-assembled nanoparticle-forming derivative of polyethylenimine
Objective(s): ShRNA-mediated silencing strategy is considered to be a potent therapeutic approach. The present study aimed to assess the ability of the previously prepared polyethylenimine (PEI) derivative for the shRNA knock-down of the CD200 gene on the cells obtained from the patients with chronic lymphocytic leukemia (CLL). Materials and Methods: Since there are several investigations...
متن کاملBiogenic amines in sea products
Sea food products are being processed through various techniques, i.e. chilling and freezing, heating, irradiating, and curing; regard to the condition of processing, formation of biogenic amines are probable, as raw materials are usually rich in respective precursors. Fermented fish products are among the most vulnerable items to this challenge, these products due to their nutritious value are...
متن کامل